Back to Build/check report for BioC 3.17:   simplified   long
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This page was generated on 2023-02-27 02:34:32 -0000 (Mon, 27 Feb 2023).

HostnameOSArch (*)R versionInstalled pkgs
kunpeng1Linux (Ubuntu 22.04.1 LTS)aarch64R Under development (unstable) (2023-01-14 r83615) -- "Unsuffered Consequences" 4259
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CHECK results for CRISPRseek on kunpeng1


To the developers/maintainers of the CRISPRseek package:
- Please allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/CRISPRseek.git to
reflect on this report. See How and When does the builder pull? When will my changes propagate? for more information.
- Make sure to use the following settings in order to reproduce any error or warning you see on this page.

raw results

Package 432/2169HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
CRISPRseek 1.39.0  (landing page)
Lihua Julie Zhu
Snapshot Date: 2023-02-23 09:40:21 -0000 (Thu, 23 Feb 2023)
git_url: https://git.bioconductor.org/packages/CRISPRseek
git_branch: master
git_last_commit: 1ce9399
git_last_commit_date: 2022-11-01 15:09:00 -0000 (Tue, 01 Nov 2022)
kunpeng1Linux (Ubuntu 22.04.1 LTS) / aarch64  OK    OK    ERROR  

Summary

Package: CRISPRseek
Version: 1.39.0
Command: /home/biocbuild/bbs-3.17-bioc/R/bin/R CMD check --install=check:CRISPRseek.install-out.txt --library=/home/biocbuild/bbs-3.17-bioc/R/library --timings CRISPRseek_1.39.0.tar.gz
StartedAt: 2023-02-24 09:38:09 -0000 (Fri, 24 Feb 2023)
EndedAt: 2023-02-24 09:49:10 -0000 (Fri, 24 Feb 2023)
EllapsedTime: 660.8 seconds
RetCode: 1
Status:   ERROR  
CheckDir: CRISPRseek.Rcheck
Warnings: NA

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.17-bioc/R/bin/R CMD check --install=check:CRISPRseek.install-out.txt --library=/home/biocbuild/bbs-3.17-bioc/R/library --timings CRISPRseek_1.39.0.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck’
* using R Under development (unstable) (2023-01-14 r83615)
* using platform: aarch64-unknown-linux-gnu (64-bit)
* R was compiled by
    gcc (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0
    GNU Fortran (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0
* running under: Ubuntu 22.04.1 LTS
* using session charset: UTF-8
* checking for file ‘CRISPRseek/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘CRISPRseek’ version ‘1.39.0’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘CRISPRseek’ can be installed ... OK
* checking installed package size ... NOTE
  installed size is 12.9Mb
  sub-directories of 1Mb or more:
    extdata  12.4Mb
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... NOTE
annotateOffTargets: no visible global function definition for ‘exons’
annotateOffTargets: no visible global function definition for
  ‘seqlevels<-’
annotateOffTargets: no visible global function definition for ‘genes’
getOfftargetScore2: no visible binding for global variable ‘subPAM’
getOfftargetScoreBulge: no visible global function definition for
  ‘import’
predictRelativeFreqIndels : <anonymous>: no visible global function
  definition for ‘predIndelFreq’
Undefined global functions or variables:
  exons genes import predIndelFreq seqlevels<- subPAM
* checking Rd files ... NOTE
prepare_Rd: annotateOffTargets.Rd:64-66: Dropping empty section \details
prepare_Rd: annotateOffTargets.Rd:67-69: Dropping empty section \note
prepare_Rd: buildFeatureVectorForScoring.Rd:86-88: Dropping empty section \details
prepare_Rd: buildFeatureVectorForScoring.Rd:89-91: Dropping empty section \note
prepare_Rd: buildFeatureVectorForScoring.Rd:99-101: Dropping empty section \references
prepare_Rd: compare2Sequences.Rd:263-265: Dropping empty section \details
prepare_Rd: compare2Sequences.Rd:266-268: Dropping empty section \note
prepare_Rd: filterOffTarget.Rd:151-153: Dropping empty section \details
prepare_Rd: filterOffTarget.Rd:154-156: Dropping empty section \note
prepare_Rd: filtergRNAs.Rd:48-50: Dropping empty section \details
prepare_Rd: filtergRNAs.Rd:51-53: Dropping empty section \note
prepare_Rd: filtergRNAs.Rd:70-72: Dropping empty section \references
prepare_Rd: findgRNAs.Rd:307-309: Dropping empty section \references
prepare_Rd: getOfftargetScore.Rd:79-81: Dropping empty section \note
prepare_Rd: isPatternUnique.Rd:23-25: Dropping empty section \details
prepare_Rd: isPatternUnique.Rd:26-28: Dropping empty section \note
prepare_Rd: isPatternUnique.Rd:37-39: Dropping empty section \references
prepare_Rd: isPatternUnique.Rd:40-42: Dropping empty section \seealso
prepare_Rd: offTargetAnalysis.Rd:438-440: Dropping empty section \details
prepare_Rd: offTargetAnalysis.Rd:441-443: Dropping empty section \note
prepare_Rd: offTargetAnalysisWithoutBSgenome.Rd:440-442: Dropping empty section \details
prepare_Rd: offTargetAnalysisWithoutBSgenome.Rd:443-445: Dropping empty section \note
prepare_Rd: searchHits.Rd:99-101: Dropping empty section \details
prepare_Rd: searchHits.Rd:102-104: Dropping empty section \note
prepare_Rd: searchHits.Rd:130-132: Dropping empty section \references
prepare_Rd: searchHits2.Rd:116-118: Dropping empty section \details
prepare_Rd: searchHits2.Rd:119-121: Dropping empty section \note
prepare_Rd: searchHits2.Rd:152-154: Dropping empty section \references
prepare_Rd: translatePattern.Rd:22-24: Dropping empty section \details
prepare_Rd: translatePattern.Rd:25-27: Dropping empty section \note
prepare_Rd: translatePattern.Rd:33-35: Dropping empty section \references
prepare_Rd: translatePattern.Rd:36-38: Dropping empty section \seealso
prepare_Rd: uniqueREs.Rd:46-48: Dropping empty section \details
prepare_Rd: uniqueREs.Rd:49-51: Dropping empty section \note
prepare_Rd: uniqueREs.Rd:61-63: Dropping empty section \references
prepare_Rd: uniqueREs.Rd:64-66: Dropping empty section \seealso
prepare_Rd: writeHits.Rd:89-91: Dropping empty section \details
prepare_Rd: writeHits.Rd:92-94: Dropping empty section \note
prepare_Rd: writeHits2.Rd:99-101: Dropping empty section \details
prepare_Rd: writeHits2.Rd:102-104: Dropping empty section \note
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... ERROR
Running examples in ‘CRISPRseek-Ex.R’ failed
The error most likely occurred in:

> base::assign(".ptime", proc.time(), pos = "CheckExEnv")
> ### Name: CRISPRseek-package
> ### Title: Design of target-specific guide RNAs (gRNAs) in CRISPR-Cas9,
> ###   genome-editing systems
> ### Aliases: CRISPRseek-package CRISPRseek
> ### Keywords: package
> 
> ### ** Examples
> 
>     library(CRISPRseek)
>     library("BSgenome.Hsapiens.UCSC.hg19")
Loading required package: BSgenome
Loading required package: GenomicRanges
Loading required package: rtracklayer
>     library(TxDb.Hsapiens.UCSC.hg19.knownGene)
Loading required package: GenomicFeatures
Loading required package: AnnotationDbi
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

>     library(org.Hs.eg.db)

>     outputDir <- getwd()
>     inputFilePath <- system.file("extdata", "inputseq.fa", package = "CRISPRseek")
>     REpatternFile <- system.file("extdata", "NEBenzymes.fa", package = "CRISPRseek")
> ######## Scenario 1. Target and off-target analysis for paired gRNAs with 
> ######## one of the pairs overlap RE sites
>     results <- offTargetAnalysis(inputFilePath, findgRNAsWithREcutOnly=TRUE,
+         REpatternFile =REpatternFile,findPairedgRNAOnly=TRUE, 
+         BSgenomeName=Hsapiens, txdb=TxDb.Hsapiens.UCSC.hg19.knownGene, 
+         orgAnn = org.Hs.egSYMBOL,max.mismatch = 1, chromToSearch = "chrX",
+         outputDir = outputDir,overwrite = TRUE)
Validating input ...
Searching for gRNAs ...
>>> Finding all hits in sequence chrX ...
>>> DONE searching
Building feature vectors for scoring ...
Calculating scores ...
Annotating, filtering and generating reports ...
Done annotating
Add paired information...
Add RE information...
write gRNAs to bed file...
Scan for REsites in flanking region...
Done. Please check output files in directory 
 /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/ 
> 
> ######## Scenario 2. Target and off-target analysis for paired gRNAs with or 
> ######## without RE sites
>     results <- offTargetAnalysis(inputFilePath, findgRNAsWithREcutOnly = FALSE,
+         REpatternFile = REpatternFile,findPairedgRNAOnly = TRUE,
+         BSgenomeName = Hsapiens, txdb = TxDb.Hsapiens.UCSC.hg19.knownGene, 
+         orgAnn = org.Hs.egSYMBOL,max.mismatch = 1, chromToSearch = "chrX",
+         outputDir = outputDir, overwrite = TRUE)
Validating input ...
Searching for gRNAs ...
>>> Finding all hits in sequence chrX ...
>>> DONE searching
Building feature vectors for scoring ...
Calculating scores ...
Annotating, filtering and generating reports ...
Done annotating
Add paired information...
Add RE information...
write gRNAs to bed file...
Scan for REsites in flanking region...
Done. Please check output files in directory 
 /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/ 
> 
> ######## Scenario 3. Target and off-target analysis for gRNAs overlap RE sites
> 
>     results <- offTargetAnalysis(inputFilePath, findgRNAsWithREcutOnly = TRUE,
+         REpatternFile = REpatternFile,findPairedgRNAOnly = FALSE, 
+         BSgenomeName = Hsapiens, txdb = TxDb.Hsapiens.UCSC.hg19.knownGene, 
+         orgAnn = org.Hs.egSYMBOL, max.mismatch = 1, chromToSearch = "chrX", 
+         outputDir = outputDir, overwrite = TRUE)
Validating input ...
Searching for gRNAs ...
>>> Finding all hits in sequence chrX ...
>>> DONE searching
Building feature vectors for scoring ...
Calculating scores ...
Annotating, filtering and generating reports ...
Done annotating
Add paired information...
Add RE information...
write gRNAs to bed file...
Scan for REsites in flanking region...
Done. Please check output files in directory 
 /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/ 
> 
> ######## Scenario 4. Off-target analysis for all potential gRNAs, this will 
> ########be the slowest among the aforementioned scenarios.
> 
>     results <- offTargetAnalysis(inputFilePath, findgRNAsWithREcutOnly = FALSE, 
+         REpatternFile = REpatternFile,findPairedgRNAOnly = FALSE,
+         BSgenomeName = Hsapiens, txdb = TxDb.Hsapiens.UCSC.hg19.knownGene, 
+          orgAnn = org.Hs.egSYMBOL, max.mismatch = 1, chromToSearch = "chrX", 
+         outputDir = outputDir,overwrite = TRUE)
Validating input ...
Searching for gRNAs ...
>>> Finding all hits in sequence chrX ...
>>> DONE searching
Building feature vectors for scoring ...
Calculating scores ...
Annotating, filtering and generating reports ...
Done annotating
Add paired information...
Add RE information...
write gRNAs to bed file...
Scan for REsites in flanking region...
Done. Please check output files in directory 
 /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/ 
> 
> ######## Scenario 5. Target and off-target analysis for gRNAs input by user.
>     gRNAFilePath <- system.file("extdata", "testHsap_GATA1_ex2_gRNA1.fa",
+         package="CRISPRseek")
>     results <- offTargetAnalysis(inputFilePath = gRNAFilePath, findgRNAs = FALSE, 
+         findgRNAsWithREcutOnly = FALSE, REpatternFile = REpatternFile,
+         findPairedgRNAOnly = FALSE, BSgenomeName = Hsapiens, 
+         txdb = TxDb.Hsapiens.UCSC.hg19.knownGene,
+          orgAnn = org.Hs.egSYMBOL, max.mismatch = 1, chromToSearch = "chrX", 
+         outputDir = outputDir, overwrite = TRUE)
Validating input ...
>>> Finding all hits in sequence chrX ...
>>> DONE searching
Building feature vectors for scoring ...
Calculating scores ...
Annotating, filtering and generating reports ...
Done annotating
Add RE information...
write gRNAs to bed file...
Scan for REsites in flanking region...
Done. Please check output files in directory 
 /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/ 
> 
> ####### Scenario 6. Quick gRNA finding  without target and off-target analysis 
>     results <- offTargetAnalysis(inputFilePath, findgRNAsWithREcutOnly = TRUE,
+         REpatternFile = REpatternFile,findPairedgRNAOnly = TRUE,  
+         chromToSearch = "", outputDir = outputDir, overwrite = TRUE)
Validating input ...
Searching for gRNAs ...
Done. Please check output files in directory  /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/ 
> 
> ####### Scenario 7. Quick gRNA finding  with gRNA efficacy analysis
>     results <- offTargetAnalysis(inputFilePath, findgRNAsWithREcutOnly = TRUE,
+         REpatternFile = REpatternFile,findPairedgRNAOnly = TRUE,
+ 	BSgenomeName = Hsapiens, annotateExon = FALSE,
+         max.mismatch = 0, outputDir = outputDir, overwrite = TRUE)
Validating input ...
Searching for gRNAs ...
>>> Finding all hits in sequence chr1 ...
>>> DONE searching
>>> Finding all hits in sequence chr2 ...
>>> DONE searching
>>> Finding all hits in sequence chr3 ...
Killed
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘runTests.R’
  Running ‘testthat.R’
 ERROR
Running the tests in ‘tests/testthat.R’ failed.
Last 13 lines of output:
  Building feature vectors for scoring ...
  Calculating scores ...
  Annotating, filtering and generating reports ...
  Done annotating
  Add RE information...
  write gRNAs to bed file...
  Scan for REsites in flanking region...
  Done. Please check output files in directory 
   /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/tests/testthat/ 
  testing gRNAs provided without filtering gRNAs with RE for offTargetAnalysis
  checking N in PAM...Testing for mouse with orgAnn...
  Validating input ...
  Searching for gRNAs ...
  >>> Finding all hits in sequence chr1 ...
  Killed
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in ‘inst/doc’ ... OK
* checking running R code from vignettes ...
  ‘CRISPRseek.Rnw’ using ‘UTF-8’... failed to complete the test
 ERROR
Errors in running code in vignettes:
when running code in ‘CRISPRseek.Rnw’
  ...
Validating input ...
Searching for gRNAs ...
>>> Finding all hits in sequence chrX ...
>>> DONE searching
Building feature vectors for scoring ...
Calculating scores ...
Annotating, filtering and generating reports ...
Killed

... incomplete output.  Crash?

* checking re-building of vignette outputs ... NOTE
Error(s) in re-building vignettes:
--- re-building ‘CRISPRseek.Rnw’ using Sweave
Loading required package: BiocGenerics

Attaching package: ‘BiocGenerics’

The following objects are masked from ‘package:stats’:

    IQR, mad, sd, var, xtabs

The following objects are masked from ‘package:base’:

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
    pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
    tapply, union, unique, unsplit, which.max, which.min

Loading required package: Biostrings
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: ‘S4Vectors’

The following objects are masked from ‘package:base’:

    I, expand.grid, unname

Loading required package: IRanges
Loading required package: XVector
Loading required package: GenomeInfoDb

Attaching package: ‘Biostrings’

The following object is masked from ‘package:base’:

    strsplit

Loading required package: BSgenome
Loading required package: GenomicRanges
Loading required package: rtracklayer
Loading required package: GenomicFeatures
Loading required package: AnnotationDbi
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.


Killed

* checking PDF version of manual ... OK
* DONE

Status: 3 ERRORs, 4 NOTEs
See
  ‘/home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/00check.log’
for details.


Installation output

CRISPRseek.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.17-bioc/R/bin/R CMD INSTALL CRISPRseek
###
##############################################################################
##############################################################################


* installing to library ‘/home/biocbuild/bbs-3.17-bioc/R/library’
* installing *source* package ‘CRISPRseek’ ...
** using staged installation
** R
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (CRISPRseek)

Tests output

CRISPRseek.Rcheck/tests/runTests.Rout


R Under development (unstable) (2023-01-14 r83615) -- "Unsuffered Consequences"
Copyright (C) 2023 The R Foundation for Statistical Computing
Platform: aarch64-unknown-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> #BiocGenerics:::testPackage("CRISPRseek")
> 
> proc.time()
   user  system elapsed 
  0.172   0.050   0.209 

CRISPRseek.Rcheck/tests/testthat.Rout.fail


R Under development (unstable) (2023-01-14 r83615) -- "Unsuffered Consequences"
Copyright (C) 2023 The R Foundation for Statistical Computing
Platform: aarch64-unknown-linux-gnu (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> Sys.setenv(R_TESTS="")
> require("CRISPRseek") || stop("unable to load Package:CRISPRseek")
Loading required package: CRISPRseek
Loading required package: BiocGenerics

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
    pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
    tapply, union, unique, unsplit, which.max, which.min

Loading required package: Biostrings
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following objects are masked from 'package:base':

    I, expand.grid, unname

Loading required package: IRanges
Loading required package: XVector
Loading required package: GenomeInfoDb

Attaching package: 'Biostrings'

The following object is masked from 'package:base':

    strsplit

[1] TRUE
> require("BSgenome.Hsapiens.UCSC.hg19") || 
+   stop("unable to load Package:BSgenome.Hsapiens.UCSC.hg19")
Loading required package: BSgenome.Hsapiens.UCSC.hg19
Loading required package: BSgenome
Loading required package: GenomicRanges
Loading required package: rtracklayer
[1] TRUE
> require("org.Hs.eg.db") || stop("unable to load Package:org.Hs.eg.db")
Loading required package: org.Hs.eg.db
Loading required package: AnnotationDbi
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.


[1] TRUE
> require("TxDb.Hsapiens.UCSC.hg19.knownGene") || 
+   stop("unable to load TxDb.Hsapiens.UCSC.hg19.knownGene")
Loading required package: TxDb.Hsapiens.UCSC.hg19.knownGene
Loading required package: GenomicFeatures
[1] TRUE
> require("BSgenome.Mmusculus.UCSC.mm10") || 
+   stop("unable to load Package: BSgenome.Mmusculus.UCSC.mm10")
Loading required package: BSgenome.Mmusculus.UCSC.mm10
[1] TRUE
> require("TxDb.Mmusculus.UCSC.mm10.knownGene") || 
+   stop("unable to load Package:TxDb.Mmusculus.UCSC.mm10.knownGene")
Loading required package: TxDb.Mmusculus.UCSC.mm10.knownGene
[1] TRUE
> require("org.Mm.eg.db") || 
+   stop("unable to load Package:org.Mm.eg.db")
Loading required package: org.Mm.eg.db

[1] TRUE
> require("testthat") || stop("unable to load testthat")
Loading required package: testthat
[1] TRUE
> test_check("CRISPRseek")
testing compare2Sequences 
search for gRNAs for input file1...
Validating input ...
Searching for gRNAs ...
Done. Please check output files in directory  /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/tests/testthat/rs362331T.fa-Feb-24-2023/ 
search for gRNAs for input file2...
Validating input ...
Searching for gRNAs ...
Done. Please check output files in directory  /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/tests/testthat/rs362331C.fa-Feb-24-2023/ 
[1] "Scoring ..."
>>> Finding all hits in sequence rs362331C ...
>>> DONE searching
finish off-target search in sequence 2
>>> Finding all hits in sequence rs362331T ...
>>> DONE searching
finish off-target search in sequence 1
finish feature vector building
finish score calculation
[1] "Done!"
search for gRNAs for input file1...
Validating input ...
Searching for gRNAs ...
Done. Please check output files in directory  /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/tests/testthat/rs362331T.fa-Feb-24-2023/ 
search for gRNAs for input file2...
Validating input ...
Searching for gRNAs ...
Done. Please check output files in directory  /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/tests/testthat/rs362331C.fa-Feb-24-2023/ 
[1] "Scoring ..."
>>> Finding all hits in sequence rs362331C ...
>>> DONE searching
finish off-target search in sequence 2
>>> Finding all hits in sequence rs362331T ...
>>> DONE searching
finish off-target search in sequence 1
finish feature vector building
finish score calculation
[1] "Done!"
search for gRNAs for input file1...
Validating input ...
Searching for gRNAs ...
Done. Please check output files in directory  /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/tests/testthat/rs362331T.fa-Feb-24-2023/ 
search for gRNAs for input file2...
Validating input ...
Searching for gRNAs ...
Done. Please check output files in directory  /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/tests/testthat/rs362331C.fa-Feb-24-2023/ 
[1] "Scoring ..."
>>> Finding all hits in sequence rs362331C ...
>>> DONE searching
finish off-target search in sequence 2
>>> Finding all hits in sequence rs362331T ...
>>> DONE searching
finish off-target search in sequence 1
finish feature vector building
finish score calculation
[1] "Done!"
search for gRNAs for input file1...
[1] "Scoring ..."
>>> Finding all hits in sequence rs362331C ...
>>> DONE searching
finish off-target search in sequence 2
finish off-target search in sequence 1
finish feature vector building
finish score calculation
[1] "Done!"
[1] "Scoring ..."
finish off-target search in sequence 2
>>> Finding all hits in sequence rs362331C ...
>>> DONE searching
finish off-target search in sequence 1
finish feature vector building
finish score calculation
[1] "Done!"
testing gRNAs provided and filter gRNAs with RE for offTargetAnalysis
Validating input ...
>>> Finding all hits in sequence chr4 ...
>>> DONE searching
Building feature vectors for scoring ...
Calculating scores ...
Annotating, filtering and generating reports ...
Done annotating
Add RE information...
write gRNAs to bed file...
Scan for REsites in flanking region...
Done. Please check output files in directory 
 /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/tests/testthat/ 
Validating input ...
>>> Finding all hits in sequence chr4 ...
>>> DONE searching
Building feature vectors for scoring ...
Calculating scores ...
Annotating, filtering and generating reports ...
Done annotating
Add RE information...
write gRNAs to bed file...
Scan for REsites in flanking region...
Done. Please check output files in directory 
 /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/tests/testthat/ 
Validating input ...
>>> Finding all hits in sequence chr4 ...
>>> DONE searching
Building feature vectors for scoring ...
Calculating scores ...
Annotating, filtering and generating reports ...
Done annotating
Add RE information...
write gRNAs to bed file...
Scan for REsites in flanking region...
Done. Please check output files in directory 
 /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/tests/testthat/ 
Validating input ...
>>> Finding all hits in sequence chr4 ...
>>> DONE searching
Building feature vectors for scoring ...
Calculating scores ...
Annotating, filtering and generating reports ...
Done annotating
Add RE information...
write gRNAs to bed file...
Scan for REsites in flanking region...
Done. Please check output files in directory 
 /home/biocbuild/bbs-3.17-bioc/meat/CRISPRseek.Rcheck/tests/testthat/ 
testing gRNAs provided without filtering gRNAs with RE for offTargetAnalysis
checking N in PAM...Testing for mouse with orgAnn...
Validating input ...
Searching for gRNAs ...
>>> Finding all hits in sequence chr1 ...
Killed

Example timings

CRISPRseek.Rcheck/CRISPRseek-Ex.timings

nameusersystemelapsed