| Back to Build/check report for BioC 3.17: simplified long | 
  | 
This page was generated on 2023-03-01 07:38:39 -0000 (Wed, 01 Mar 2023).
| Hostname | OS | Arch (*) | R version | Installed pkgs | 
|---|---|---|---|---|
| kunpeng1 | Linux (Ubuntu 22.04.1 LTS) | aarch64 | R Under development (unstable) (2023-01-14 r83615) -- "Unsuffered Consequences" | 4266 | 
| Click on any hostname to see more info about the system (e.g. compilers) (*) as reported by 'uname -p', except on Windows and Mac OS X | ||||
| 
To the developers/maintainers of the DESeq2 package: - Please allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/DESeq2.git to reflect on this report. See How and When does the builder pull? When will my changes propagate? for more information. - Make sure to use the following settings in order to reproduce any error or warning you see on this page.  | 
| Package 514/2171 | Hostname | OS / Arch | INSTALL | BUILD | CHECK | BUILD BIN | ||||||||
| DESeq2 1.39.6  (landing page) Michael Love 
  | kunpeng1 | Linux (Ubuntu 22.04.1 LTS) / aarch64 | OK | OK | ERROR | |||||||||
| Package: DESeq2 | 
| Version: 1.39.6 | 
| Command: /home/biocbuild/bbs-3.17-bioc/R/bin/R CMD check --install=check:DESeq2.install-out.txt --library=/home/biocbuild/bbs-3.17-bioc/R/library --timings DESeq2_1.39.6.tar.gz | 
| StartedAt: 2023-02-28 01:44:39 -0000 (Tue, 28 Feb 2023) | 
| EndedAt: 2023-02-28 01:54:44 -0000 (Tue, 28 Feb 2023) | 
| EllapsedTime: 604.8 seconds | 
| RetCode: 1 | 
| Status: ERROR | 
| CheckDir: DESeq2.Rcheck | 
| Warnings: NA | 
##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.17-bioc/R/bin/R CMD check --install=check:DESeq2.install-out.txt --library=/home/biocbuild/bbs-3.17-bioc/R/library --timings DESeq2_1.39.6.tar.gz
###
##############################################################################
##############################################################################
* using log directory ‘/home/biocbuild/bbs-3.17-bioc/meat/DESeq2.Rcheck’
* using R Under development (unstable) (2023-01-14 r83615)
* using platform: aarch64-unknown-linux-gnu (64-bit)
* R was compiled by
    gcc (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0
    GNU Fortran (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0
* running under: Ubuntu 22.04.1 LTS
* using session charset: UTF-8
* checking for file ‘DESeq2/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘DESeq2’ version ‘1.39.6’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘DESeq2’ can be installed ... OK
* used C++ compiler: ‘g++ (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0’
* checking installed package size ... OK
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking line endings in C/C++/Fortran sources/headers ... OK
* checking line endings in Makefiles ... OK
* checking compilation flags in Makevars ... OK
* checking for GNU extensions in Makefiles ... OK
* checking for portable use of $(BLAS_LIBS) and $(LAPACK_LIBS) ... OK
* checking use of PKG_*FLAGS in Makefiles ... OK
* checking compiled code ... NOTE
Note: information on .o files is not available
* checking files in ‘vignettes’ ... OK
* checking examples ... ERROR
Running examples in ‘DESeq2-Ex.R’ failed
The error most likely occurred in:
> base::assign(".ptime", proc.time(), pos = "CheckExEnv")
> ### Name: unmix
> ### Title: Unmix samples using loss in a variance stabilized space
> ### Aliases: unmix
> 
> ### ** Examples
> 
> 
> # some artificial data
> cts <- matrix(c(80,50,1,100,
+                 1,1,60,100,
+                 0,50,60,100), ncol=4, byrow=TRUE)
> # make a DESeqDataSet
> dds <- DESeqDataSetFromMatrix(cts,
+   data.frame(row.names=seq_len(ncol(cts))), ~1)
converting counts to integer mode
> colnames(dds) <- paste0("sample",1:4)
> 
> # note! here you would instead use
> # estimateSizeFactors() to do actual normalization
> sizeFactors(dds) <- rep(1, ncol(dds))
> 
> norm.cts <- counts(dds, normalized=TRUE)
> 
> # 'pure' should also have normalized counts...
> pure <- matrix(c(10,0,0,
+                  0,0,10,
+                  0,10,0), ncol=3, byrow=TRUE)
> colnames(pure) <- letters[1:3]
> 
> # for real data, you need to find alpha after fitting estimateDispersions()
> mix <- unmix(norm.cts, pure, alpha=0.01)
Warning in sqrt(alpha * q) : NaNs produced
Error in optim(par = rep(1, ncol(pure)), fn = sumLossVST, gr = NULL, i,  : 
  L-BFGS-B needs finite values of 'fn'
Calls: unmix -> lapply -> lapply -> FUN -> optim
Execution halted
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘testthat.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in ‘inst/doc’ ... OK
* checking running R code from vignettes ...
  ‘DESeq2.Rmd’ using ‘UTF-8’... OK
 NONE
* checking re-building of vignette outputs ... OK
* checking PDF version of manual ... OK
* DONE
Status: 1 ERROR, 1 NOTE
See
  ‘/home/biocbuild/bbs-3.17-bioc/meat/DESeq2.Rcheck/00check.log’
for details.
DESeq2.Rcheck/00install.out
############################################################################## ############################################################################## ### ### Running command: ### ### /home/biocbuild/bbs-3.17-bioc/R/bin/R CMD INSTALL DESeq2 ### ############################################################################## ############################################################################## * installing to library ‘/home/biocbuild/bbs-3.17-bioc/R/library’ * installing *source* package ‘DESeq2’ ... ** using staged installation ** libs using C++ compiler: ‘g++ (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0’ g++ -std=gnu++14 -I"/home/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG -I'/home/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'/home/biocbuild/bbs-3.17-bioc/R/library/RcppArmadillo/include' -I/usr/local/include -fPIC -g -O2 -Wall -c DESeq2.cpp -o DESeq2.o g++ -std=gnu++14 -I"/home/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG -I'/home/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'/home/biocbuild/bbs-3.17-bioc/R/library/RcppArmadillo/include' -I/usr/local/include -fPIC -g -O2 -Wall -c RcppExports.cpp -o RcppExports.o g++ -std=gnu++14 -shared -L/home/biocbuild/bbs-3.17-bioc/R/lib -L/usr/local/lib -o DESeq2.so DESeq2.o RcppExports.o -llapack -L/home/biocbuild/bbs-3.17-bioc/R/lib -lRblas -lgfortran -lm -L/home/biocbuild/bbs-3.17-bioc/R/lib -lR installing to /home/biocbuild/bbs-3.17-bioc/R/library/00LOCK-DESeq2/00new/DESeq2/libs ** R ** inst ** byte-compile and prepare package for lazy loading ** help *** installing help indices ** building package indices ** installing vignettes ** testing if installed package can be loaded from temporary location ** checking absolute paths in shared objects and dynamic libraries ** testing if installed package can be loaded from final location ** testing if installed package keeps a record of temporary installation path * DONE (DESeq2)
DESeq2.Rcheck/tests/testthat.Rout
R Under development (unstable) (2023-01-14 r83615) -- "Unsuffered Consequences"
Copyright (C) 2023 The R Foundation for Statistical Computing
Platform: aarch64-unknown-linux-gnu (64-bit)
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library("testthat")
> library("DESeq2")
Loading required package: S4Vectors
Loading required package: stats4
Loading required package: BiocGenerics
Attaching package: 'BiocGenerics'
The following objects are masked from 'package:stats':
    IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
    pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
    tapply, union, unique, unsplit, which.max, which.min
Attaching package: 'S4Vectors'
The following objects are masked from 'package:base':
    I, expand.grid, unname
Loading required package: IRanges
Loading required package: GenomicRanges
Loading required package: GenomeInfoDb
Loading required package: SummarizedExperiment
Loading required package: MatrixGenerics
Loading required package: matrixStats
Attaching package: 'MatrixGenerics'
The following objects are masked from 'package:matrixStats':
    colAlls, colAnyNAs, colAnys, colAvgsPerRowSet, colCollapse,
    colCounts, colCummaxs, colCummins, colCumprods, colCumsums,
    colDiffs, colIQRDiffs, colIQRs, colLogSumExps, colMadDiffs,
    colMads, colMaxs, colMeans2, colMedians, colMins, colOrderStats,
    colProds, colQuantiles, colRanges, colRanks, colSdDiffs, colSds,
    colSums2, colTabulates, colVarDiffs, colVars, colWeightedMads,
    colWeightedMeans, colWeightedMedians, colWeightedSds,
    colWeightedVars, rowAlls, rowAnyNAs, rowAnys, rowAvgsPerColSet,
    rowCollapse, rowCounts, rowCummaxs, rowCummins, rowCumprods,
    rowCumsums, rowDiffs, rowIQRDiffs, rowIQRs, rowLogSumExps,
    rowMadDiffs, rowMads, rowMaxs, rowMeans2, rowMedians, rowMins,
    rowOrderStats, rowProds, rowQuantiles, rowRanges, rowRanks,
    rowSdDiffs, rowSds, rowSums2, rowTabulates, rowVarDiffs, rowVars,
    rowWeightedMads, rowWeightedMeans, rowWeightedMedians,
    rowWeightedSds, rowWeightedVars
Loading required package: Biobase
Welcome to Bioconductor
    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.
Attaching package: 'Biobase'
The following object is masked from 'package:MatrixGenerics':
    rowMedians
The following objects are masked from 'package:matrixStats':
    anyMissing, rowMedians
> test_check("DESeq2")
[ FAIL 0 | WARN 1 | SKIP 3 | PASS 232 ]
══ Skipped tests ═══════════════════════════════════════════════════════════════
• empty test (3)
[ FAIL 0 | WARN 1 | SKIP 3 | PASS 232 ]
> 
> proc.time()
   user  system elapsed 
257.443   5.441 261.521 
DESeq2.Rcheck/DESeq2-Ex.timings
| name | user | system | elapsed | |
| DESeq | 6.932 | 0.192 | 7.124 | |
| DESeqDataSet | 0.270 | 0.000 | 0.269 | |
| coef | 2.855 | 0.072 | 2.927 | |
| collapseReplicates | 0.465 | 0.012 | 0.478 | |
| counts | 0.420 | 0.004 | 0.424 | |
| design | 0.228 | 0.000 | 0.228 | |
| dispersionFunction | 2.107 | 0.056 | 2.162 | |
| estimateDispersions | 1.473 | 0.007 | 1.482 | |
| estimateDispersionsGeneEst | 1.529 | 0.032 | 1.561 | |
| estimateSizeFactors | 1.064 | 0.007 | 1.071 | |
| estimateSizeFactorsForMatrix | 0.232 | 0.009 | 0.240 | |
| fpkm | 0.997 | 0.008 | 1.006 | |
| fpm | 0.753 | 0.020 | 0.772 | |
| integrateWithSingleCell | 0 | 0 | 0 | |
| lfcShrink | 4.544 | 0.046 | 4.591 | |
| makeExampleDESeqDataSet | 0.213 | 0.001 | 0.213 | |
| nbinomLRT | 2.469 | 0.020 | 2.489 | |
| nbinomWaldTest | 2.416 | 0.027 | 2.444 | |
| normalizationFactors | 2.503 | 0.036 | 2.539 | |
| plotCounts | 0.532 | 0.001 | 0.532 | |
| plotDispEsts | 1.566 | 0.008 | 1.574 | |
| plotMA | 2.873 | 0.012 | 2.884 | |
| plotPCA | 2.626 | 0.040 | 2.665 | |
| plotSparsity | 0.493 | 0.000 | 0.494 | |
| results | 10.870 | 0.111 | 10.982 | |
| rlog | 1.384 | 0.016 | 1.400 | |
| summary | 2.969 | 0.017 | 2.986 | |